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KMID : 0613820190290020198
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Journal of Life Science
2019 Volume.29 No. 2 p.198 ~ p.201
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Determination of Siderophore from Bacillus Mojavensis Using Liquid Chromatography quadrupole Time-of-flight Tandem Mass Spectrometry
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Cheon Hae-In
Yeo Mi-Seon
Kim Kang-Min
Kang Jae-Seon
Pyo Jae-Sung
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Abstract
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Recently, it has been reported that Bacillus mojavensis possesses antifungal properties and plant growth- promoting activities, which are similar to the characteristics of siderophore. In this study, the siderophore produced by B. mojavensis was assessed using a solid phase extraction (SPE) cartridge and liquid chromatography quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS). After B. mojavensis was incubated in phenol medium for 16 hr and lyophilized, the sample was dissolved in water and loaded to an SPE cartridge to remove interferences. The cartridge was washed with 5% methanol in water and eluted with 2% formic acid in methanol sequentially. The eluted solution was evaporated under a stream of nitrogen gas and reconstituted in methanol. The reconstituted sample was filtered, and 1 μl of the sample was assessed using Q-TOF MS/MS. The mass spectrometer was operated using the positive electrospray ionization mode. Based on the mass spectrum and tandem mass spectrum, the siderophore produced by B. mojavensis was bacillibactin, one of the catechol types of siderophore with a molecular weight of 882.2556. This siderophore analysis could provide a justification for the study of B. mojavensis as a functional food and for pharmaceutical applications.
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KEYWORD
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Bacillibactin, Bacillus mojavensis, liquid chromatography-quadrupole time-of-flight mass spectrometry, siderophore
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